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Over‐expression of natural and variant human H‐chain ferritins in E. coli
Author(s) -
Prozzi Deborah,
Crichton Robert R.,
Davison John
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81303-6
Subject(s) - ferritin , protein subunit , microbiology and biotechnology , biology , immunoglobulin light chain , biochemistry , chemistry , escherichia coli , gene , antibody , genetics
The natural human H‐chain ferritin was expressed in E. coli using a multi‐copy expression vector containing the λ p L promoter. A variant H‐ferritin, having an altered N‐terminus, was also produced. These proteins are overproduced (> 30% of the soluble protein), correctly assembled into its 24‐subunit shell, and able to bind iron. The identity of the products was confirmed using an antibody specific for H‐ferritin.