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Molecular cloning and sequencing of the chicken smooth muscle myosin regulatory light chain
Author(s) -
Messer Neil G.,
Kendrick-Jones John
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81300-0
Subject(s) - gizzard , complementary dna , immunoglobulin light chain , cdna library , myosin light chain kinase , myosin , molecular cloning , cloning (programming) , biology , troponin c , microbiology and biotechnology , gene , coding region , genetics , biochemistry , troponin , antibody , psychology , paleontology , psychiatry , myocardial infarction , computer science , programming language
A cDNA probe was constructed from a chicken skeletal muscle regulatory light chain cDNA and was used to screen a chicken gizzard cDNA library. A clone containing the entire coding region of the chicken gizzard regulatory light chain was isolated and sequenced. The deduced protein sequence is identical to the most recently reported chemical sequence of the chicken smooth muscle regulatory light chain, and has homologies with other troponin C‐like calcium‐binding proteins.