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Meso‐chlorination of chlorophyll a in the course of pigment extraction
Author(s) -
Kobayashi Masami,
Watanabe Tadashi,
Struck Andreas,
Scheer Hugo
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81282-1
Subject(s) - pigment , extraction (chemistry) , chemistry , chlorophyll , chloride , chromatography , chlorophyll a , fraction (chemistry) , nuclear chemistry , carbon fibers , environmental chemistry , organic chemistry , biochemistry , materials science , composite number , composite material
HPLC analysis has shown that the meso δ‐carbon of chlorophyll (Chl) a undergoes chlorination to give δ‐chloro Chl a (Cl‐Chl a ), possibly by an enzymatic reaction with chloride ion in the wet medium of crushed plant tissue. A sizeable amount, occasionally a 1% fraction, of the whole Chl a is chlorinated during tissue crushing/pigment extraction. The chlorination can be neatly or completely suppressed if plant materials are kneaded with a sufficient amount of a desiccant or with Cl − ‐scavenging silver ion before pigment extraction.