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The antimycin sensitivity of flash‐induced ATP synthesis in photosystem I‐enriched subchloroplast vesicles
Author(s) -
de Wolf F.A.,
Galmiche J.M.,
Kraayenhof R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81279-1
Subject(s) - antimycin a , chemistry , photophosphorylation , plastoquinone , nigericin , photosystem i , vesicle , dcmu , oligomycin , photosystem ii , photochemistry , biophysics , biochemistry , electron transport chain , thylakoid , chloroplast , photosynthesis , biology , membrane , enzyme , atpase , gene
Photosystem I‐enriched vesicles, derived from spinach thylakoids by mild digitonin treatment, reconstituted with native ferredoxin and redox‐poised with NADPH and oxygen, display antimycin‐sensitive cyclic photophosphorylation. The effects of 1–30 μM antimycin point to electron transfer inhibition: (i) they are cancelled after addition of 10 μM pyocyanin and (ii) they resemble the effects of 1–5 μM DBMIB, but differ from those of FCCP, nigericin or valinomycin. Some vesicle preparations fails to show antimycin sensitivity in the absence of pyocyanin [(1985) FEBS Lett. 192, 271–274] and have apparently lost the antimycin‐sensitive component.