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A rapid purification method for calf thymus casein kinase II
Author(s) -
Zandomeni R.,
Zandomeni M.C.,
Weinmann R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81272-9
Subject(s) - fast protein liquid chromatography , casein kinase 2 , casein , chemistry , chromatography , casein kinase 1 , kinase , protein kinase a , biochemistry , casein kinase 2, alpha 1 , nucleotide , microbiology and biotechnology , enzyme , biology , mitogen activated protein kinase kinase , gene
We report here the rapid purification to homogeneity of a cyclic nucleotide‐independent protein kinase sensitive to 5,6‐dichloro‐1‐β‐D‐ribofuranozylbenzimidazole (DRB), identical to the previously described casein kinase II, from lyophilized calf thymus by chromatography on phosphocellulose and Mono‐Q FPLC columns.