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Asn I: A novel class II restriction endonuclease from Arthrobacter sp., strain N‐CM, recognizing 5′‐AT/TAAT‐3′
Author(s) -
Rexer Bernhard U.,
Jarsch Michael,
Sagmeister Christine,
Glück Barbara,
Berger Gerhard,
Kessler Christoph
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81271-7
Subject(s) - restriction enzyme , pbr322 , plasmid , biology , strain (injury) , complementation , restriction map , genetics , endonuclease , eubacterium , arthrobacter , multiple cloning site , palindromic sequence , enzyme , dna , microbiology and biotechnology , gene , biochemistry , palindrome , recombinant dna , genome , phenotype , bacteria , vector (molecular biology) , anatomy
A new class II restriction endonuclease, Asn I, with a novel sequence specificity was isolated from the Gram‐positive eubacterium Arthrobacter species, strain N‐CM. Asn I recognizes the unambiguously defined palindromic hexanucleotideconsisting of A‐ and T‐residues. The novel enzyme in the presence of Mg 2+ cleaves specifically both strands as indicated by the arrows. The staggered cuts generate 5′‐protruding ends with single‐stranded 5′‐TA‐3′ dinucleotide extensions. The novel enzyme may be a useful tool for cloning experiments by complementation of the few enzymes such as Pst I and Pvu I cutting only once in the Amp r ‐gene of plasmids pBR322 and pBR328.