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Differences in the organization of adenovirus E1A promoters are not important for their full activity
Author(s) -
Leite José Paulo G.,
D'Halluin Jean-Claude
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81269-9
Subject(s) - plasmid , promoter , gene , transcription (linguistics) , hela , biology , coding region , adenoviridae , genetics , microbiology and biotechnology , recombinant dna , gene expression , cell culture , philosophy , linguistics
The 5′‐E1A control regions of adenovirus (Ad) types 2, 3 and 12 were cloned upstream from the cat gene. Each serotype was characterized by unique organization of the 5′‐E1A non‐coding region. All three 5′‐non‐coding E1A regions were able to stimulate the cat gene transcription in HeLa cells. Hybrid plasmids between Ad2/Ad3 and Ad2/Ad12 revealed the same level of CAT activity as the native plasmids but the optimum activity of promoter was achieved only when its organization was not modified. These observations suggest that various nuclear factors take part in the activation process of each promoter.