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Effect of exogenously added acylphosphatases on inositol lipid metabolism in human platelets
Author(s) -
Berti Andrea,
Stefani Maurizio,
Degl'Innocenti Donatella,
Ruggiero Marco,
Chiarugi Vincenzo,
Ramponi Giampiero
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81268-7
Subject(s) - phosphatidylinositol , dephosphorylation , platelet , inositol , biochemistry , chemistry , metabolism , intracellular , biology , enzyme , kinase , phosphatase , receptor , immunology
In this paper we demonstrate that human platelets contain an acylphosphatase isoenzyme. We then investigated the of exogenously added human muscle and erythrocyte acylphosphatases on inositol lipid content in human platelets permeabilized with saponin. Alterations in the level of the polyphosphoinositides were observed: in particular, the levels of phosphatidylinositol 4,5‐bisphosphate, and of phosphatidylinositol 4‐monophosphate were decreased, whereas the level of phosphatidylinositol was increased. These results suggest that acylphosphatases promote polyphosphoinositide dephosphorylation, possibly through intracellular Ca 2+ mobilization.