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Ongoing protein synthesis needed for 1,25‐(OH) 2 D 3 ‐mediated rapid increase of cyclic GMP in human skin fibroblasts
Author(s) -
Barsony Julia,
Marx Stephen J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81263-8
Subject(s) - cycloheximide , protein biosynthesis , stimulation , protein synthesis inhibitor , receptor , intracellular , diphtheria toxin , sodium nitroprusside , chemistry , endocrinology , medicine , biology , biochemistry , toxin , nitric oxide
Recently we reported that 1,25‐dihydroxyvitamin D 3 (1,25‐(OH) 2 D 3 ) through interaction with its specific receptor rapidly (within 1 min) stimulated intracellular cGMP production in cultured human skin fibroblasts. Here we show that this effect of 100 nM 1,25‐(OH) 2 D 3 is prevented by brief (30 min) inhibition of RNA synthesis (with actinomycin D or α‐amanitin) or by brief inhibition of protein synthesis (with cycloheximide or diphtheria toxin). The protein synthesis inhibitors also blocked stimulation of cGMP by other steroids (testosterone or dexamethasone at 100 nM) but did not block cGMP stimulation by sodium nitroprusside. Since the time for the 1,25‐(OH) 2 D 3 receptor to increase cGMP seems too short to require de novo protein synthesis, we conclude that the 1,25‐(OH) 2 D 3 receptor acts together with rapidly turning over protein(s) to stimulate cGMP synthesis.