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Simple graphical methods for use with complex ligand‐binding and enzyme mechanisms
Author(s) -
Crabbe M.James C.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81259-6
Subject(s) - chemistry , ligand (biochemistry) , glyceraldehyde , polyol , enzyme , dehydrogenase , simple (philosophy) , mechanism (biology) , computational chemistry , stereochemistry , biological system , biochemistry , organic chemistry , biology , physics , philosophy , epistemology , quantum mechanics , receptor , polyurethane
Simple graphical plots in distribution‐free space are shown to provide estimates of mechanism and kinetic parameters for complex steady‐state processes. The methods were tested using simulated 1:1, 1:2, 2:2, 2:3 and 3:3 functions, and real data from polyol dehydrogenase, NADPH oxidation in the presence of DL‐glyceraldehyde, and NADPH oxidation in the presence of an NADPH‐binding protein. Estimates of mechanism and parameters agreed closely with those found from non‐linear regression by computer.