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Uranyl salts as photochemical agents for cleavage of DNA and probing of protein‐DNA contacts
Author(s) -
Nielsen Peter E.,
Jeppesen Claus,
Buchardt Ole
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81245-6
Subject(s) - uranyl , uranyl acetate , dna , chemistry , uranyl nitrate , cleavage (geology) , photochemistry , repressor , biochemistry , materials science , gene , uranium , organic chemistry , biology , ion , genetics , fracture (geology) , transcription factor , metallurgy , composite material , staining
Single‐strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long‐wavelength (λ ∼ 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the λ‐repressor/O R1 operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.