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NMR‐invisible lactate in blood plasma
Author(s) -
Bell Jimmy D.,
Brown Judith C.C.,
Kubal Gina,
Sadler Peter J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81238-9
Subject(s) - spectral line , chemistry , plasma , carboxylate , nuclear magnetic resonance , glycoprotein , nmr spectra database , analytical chemistry (journal) , stereochemistry , biochemistry , chromatography , physics , quantum mechanics , astronomy
Resonances for lactate are broadened in 500 MHz 1 H NMR spectra of human blood plasma and only about one‐third is visible in Hahn spin‐echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high‐ M r fraction of plasma can give rise to peaks which are too broad to observe in either single‐pulse or spin‐echo spectra. Addition of agents such as NH 4 Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.