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Excitation‐contraction coupling in skeletal muscle fibers injected with the InsP 3 blocker, heparin
Author(s) -
Pape P.C.,
Konishi M.,
Baylor S.M.,
Somlyo A.P.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81233-x
Subject(s) - biophysics , skeletal muscle , endoplasmic reticulum , chemistry , contraction (grammar) , muscle contraction , inositol trisphosphate , inositol , heparin , endocrinology , biochemistry , biology , receptor
Heparin, an inhibitor of inositol trisphosphate (InsP 3 )‐induced Ca 2+ release in smooth muscle and non‐muscle cells, was injected into intact frog skeletal muscle fibres. Ca 2+ release from the sarcoplasmic reticulum was elicited by the normal action potential mechanism and monitored by both fura‐2 fluorescence and an intrinsic birefringence signal. Both optical signals, and hence Ca 2+ release, were unaffected by high concentrations of heparin. This result argues against a major physiological role of InsP 3 as a chemical messenger of excitation‐contraction coupling in skeletal muscle.