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Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum
Author(s) -
Bleß Wolfgang,
Barz Wolfgang
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81231-6
Subject(s) - phytoalexin , elicitor , enzyme , biochemistry , biosynthesis , suspension culture , enzyme assay , chemistry , yeast , cell culture , biology , genetics , resveratrol
Chickpea ( Cicer arietinum L.) cell suspension cultures transferred into a medium containing yeast extract as elicitor accumulate the phytoalexins medicarpin and maackiain. Concomitant with the accumulation of the pterocarpans, a new enzyme activity is induced which catalyses the conversion of the 2′‐hydroxyisoflavanone vestitone to the pterocarpan medicarpin. Maximum enzyme activity was reached 12 h after transfer of cells and then activity declined. The soluble enzyme was partially purified and characterized as pterocarpan synthase.