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The ribosomes of Aspergillus giganteus are sensitive to the cytotoxic action of α‐sarcin
Author(s) -
Miller Stephen P.,
Bodley James W.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81162-1
Subject(s) - ribosome , ribosomal rna , biology , ricin , ribosome inactivating protein , biochemistry , toxin , rna , gene
Ribosomes in lysates prepared from the mycelia of Aspergillus giganteus MDH 18894, which are actively secreting α‐sarcin, do not contain the α‐sarcin lesion. However, the addition of exogenous α‐sarcin to these same lysates results in cleavage of the 26 S rRNA of the 60 S ribosomal subunit, characteristic of the cytotoxic action of α‐sarcin. We conclude that A. giganteus ribosomes are not inherently resistant to the action of α‐sarcin but are protected in vivo by producing α‐sarcin in an inactive form and/or by the efficient cotranslational secretion of the toxin.