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Complete cDNA sequence of a Dictyostelium ubiquitin with a carboxy‐terminal tail and identification of the protein using an anti‐peptide antibody
Author(s) -
Müller-Taubenberger A.,
Westphal M.,
Jaeger E.,
Noegel A.,
Gerisch G.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81139-6
Subject(s) - dictyostelium discoideum , complementary dna , biology , peptide sequence , microbiology and biotechnology , saccharomyces cerevisiae , gene , biochemistry , ubiquitin , amino acid , messenger rna
The complete sequence of a Dictyostelium discoideum cDNA is presented that codes for monoubiquitin extended at its C‐terminus by a 52 amino acid tail. The sequence of both the ubiquitin portion and the tail is highly homologous to the one of Saccharomyces cerevisiae and to a partial mouse sequence. The highly basic tail sequence contains a putative metal and nucleic acid‐binding motif. The gene encoding the 0.6 kb mRNA of the C‐terminally extended ubiquitin is represented only once in the haploid genome. The 0.6 kb mRNA as well as its translation product, a 15 kDa protein, is expressed in exponentially growing cells and remains present for at least 5 h of development. Using antibodies against a synthetic peptide that corresponds to the C‐terminal amino acid sequence, a 15 kDa protein containing the extension was also detected in yeast.