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2‐Mercaptoethylamine, a competitive inhibitor of spermidine synthase in mammalian cells
Author(s) -
Hibasami Hiroshige,
Kawase Masahiro,
Tsukada Tetsuya,
Maekawa Satoru,
Sakurai Masaki,
Nakashima Kunio
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81133-5
Subject(s) - spermidine , putrescine , spermine , biochemistry , chemistry , polyamine , non competitive inhibition , atp synthase , k562 cells , enzyme , biology , cell
Spermidine synthase from rat ventral prostate was inhibited by 2‐mercaptoethylamine (MEA). Inhibition of spermidine synthase by MEA was competitive with respect to one of the substrates putrescine, but not competitive with respect to the other substrate decarboxylated S ‐adenosylmethionine. MEA markedly depressed spermidine and spermine contents in human erythroid leukemia K562 cells, suggesting that these changes resulted from the inhibitory effect of MEA on spermidine synthase in situ.