Premium
Sulfated N ‐linked carbohydrate chains in porcine thyroglobulin
Author(s) -
Kamerling Johannis P.,
Rijkse Irma,
Maas Augustinus A.M.,
van Kuik J.Albert,
Vliegenthart Johannes F.G.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81070-6
Subject(s) - oligosaccharide , beta (programming language) , alpha (finance) , carbohydrate , chemistry , sulfation , thyroglobulin , carbohydrate conformation , nuclear magnetic resonance spectroscopy , biochemistry , chromatography , stereochemistry , biology , medicine , antibody , immunology , construct validity , nursing , computer science , patient satisfaction , programming language
N ‐linked carbohydrate chains of porcine thyroglobulin were released by the hydrazinolysis procedure. The resulting mixture of oligosaccharide‐alditols was fractionated by high‐voltage paper electrophoresis, the acidic fractions were further separated by high‐performance liquid chromatography on Lichrosorb‐NH 2 , and analyzed by 500‐MHz 1 H‐NMR spectroscopy and, partially, by permethylation analysis. Of the acidic oligosaccharide‐alditols, the following sulfated carbohydrate chains could be identified: