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Identification of Eco RV fragments spanning the N α‐tubulin gene of Physarum
Author(s) -
Watts David I.,
Monteiro Mervyn J.,
Cox Robert A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81067-6
Subject(s) - physarum polycephalum , gene , biology , microbiology and biotechnology , coding region , genetics , ploidy , restriction fragment , dna , physarum , fragment (logic) , biochemistry , computer science , programming language
The N α‐tubulin gene of Physarum polycephalum has an Eco RV site at codons 252/253. Eco RV digestion of Physarum DNA generated two Eco RV fragments per gene copy comprising both coding and flanking sequences. Hybridisation probes which included coding sequences upstream from the central Eco RV site cross‐hybridised with another α‐tubulin gene. Probes derived from either 5′‐ or 3′‐flanking regions were gene‐specific. These probes identified two Eco RV fragments in the haploid strain CLdAXE viz 5.4 kb (5′‐fragment) and 6.2 kb (3′‐fragment). The same two fragments were identified in Eco RV digests of DNA of the diploid strain M 3 CVIII, and a second form of the gene was also identified comprising two fragments viz 5.0 kb (5′‐end) and 5.5 kb (3′‐end). Both forms gave rise to an identical 4.65 kb Hin dIII fragment as judged by restriction mapping.