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Partial sequence of the rat heavy neurofilament polypeptide (NF‐H) Identification of putative phosphorylation sites
Author(s) -
Breen Kieran C.,
Robinson Philip A.,
Wion Didier,
Anderton Brian H.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81064-0
Subject(s) - neurofilament , phosphorylation , clone (java method) , microbiology and biotechnology , complementary dna , homology (biology) , biology , peptide sequence , messenger rna , sequence (biology) , chemistry , biochemistry , amino acid , gene , immunohistochemistry , immunology
A 3 kb cDNA clone has previously been isolated in this laboratory corresponding to the rat heavy neurofilament polypeptide (NF‐H). This clone, equivalent to approximately 70% of the total mRNA of the protein has been sequenced and shown to contain the carboxy‐terminal region of the message. This contains 51 of the Lys‐Ser‐Pro repeat triplets which are reported to be the site of neurofilament phosphorylation. The sequence obtained was subsequently compared to those of mouse and human NF‐H, showing a homology of approximately 85%. There is, however, one region which is variable between the species, this being the highly phosphorylated region of the protein containing the Lys‐Ser‐Pro triplet repeat.