Premium
Oxidation of rat insulin II, but not I, leads to anomalous elution profiles upon HPLC analysis of insulin‐related peptides
Author(s) -
Gross David,
Skvorak Anne,
Hendrick Grant,
Weir Gordon,
Villa-Komaroff Lydia,
Halban Philippe
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81062-7
Subject(s) - insulin , proinsulin , elution , high performance liquid chromatography , chemistry , chromatography , medicine , endocrinology , biology
Rat insulin II, unlike rat insulin I and other non‐rodent insulins, contains a unique methionine residue at position B29. Reversed‐phase HPLC allows for separation of the two rat insulins, with insulin I typically eluting faster than insulin II. An anomalous peak of insulin immunoreactive material was found eluting even faster than insulin I following acid extraction of rat insulin‐producing cells. This early peak co‐eluted with [Met‐ O B29 ]insulin II suggesting that during cell extraction and subsequent purification steps, rat insulin II is subject to selective oxidation at Met B29 . Such oxidation of rat proinsulin II affords improved separation from rat proinsulin I compared to the native form.