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Adhesion defect of ascites cells corrected with membrane‐bound attachment molecules
Author(s) -
Gordeeva L.V.,
Bogdanov A.A.,
Baibakov B.A.,
Torchilin V.P.,
Margolis L.B.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81057-3
Subject(s) - adhesion , avidin , ascites , cell adhesion , cell adhesion molecule , biotin , microbiology and biotechnology , biophysics , cell , chemistry , cell membrane , membrane , in vitro , biology , biochemistry , medicine , organic chemistry
Adhesion is obligatory for cell proliferation in most types of cells. This function becomes defective after malignant transformation. An extreme example is ascites cells which proliferate in suspension. The nature of their defects remains obscure. Here we show that the linking of biotin molecules to the Ehrlich ascites carcinoma cells enables these cells to spread normally on an avidin‐coated substrate. The spreading was the result of specific avidin‐biotin interaction. The morphology of the spread cells and sensitivity to different inhibitors are similar to those of normal epithelia. Thus it is enough to supply appropriate substrate‐adhesive molecules to the ascites cell surface to normalize their adhesion.