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Serum induces the immediate opening of Ca 2+ ‐activated channels in quiescent human fibroblasts
Author(s) -
Peres Antonio,
Zippel Renata,
Sturani Emmapaola
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81052-4
Subject(s) - conductance , ionophore , intracellular , biophysics , membrane potential , membrane , permeability (electromagnetism) , chemistry , biochemistry , biology , physics , condensed matter physics
Application of fetal calf serum to quiescent human fibroblasts produces an immediate (3 – 20 s delay) increase in membrane conductance which lasts about 20 – 30 s. This conductance is strongly outwardly‐rectifying and has a reversal potential between −45 and −10 mV. The conductance increase may also be induced by application of the Ca 2+ ionophore A23187 while it does not occur when intracellular K + is replaced by Cs + . It is concluded that this early effect of serum is due to the opening of Ca 2+ ‐activated channels. This permeability change will alter the membrane potential and thus possibly interact with other voltage‐sensitive processes induced by serum growth factors.