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Metallothionein RNA levels in HL‐60 cells Effect of cadmium, differentiation, and protein kinase C activation
Author(s) -
Hanke Tomas,
Tyers Michael,
Harley Calvin B.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81051-2
Subject(s) - metallothionein , microbiology and biotechnology , gene expression , rna , protein kinase c , cellular differentiation , biology , transcription (linguistics) , cell culture , chemistry , thp1 cell line , gene , kinase , biochemistry , genetics , linguistics , philosophy
Metallothionein (MT) gene transcription is regulated in a developmental and tissue‐specific manner by metal ions and other agents. We examined MT expression in the human promyelocytic leukemia cell line HL‐60 during differentiation along macrophage and neutrophil lineages. All HL‐60 phenotypes showed similar basal levels of MT RNA with significant induction following exposure to Cd 2+ but not activators of PKC. MT RNA did not correlate with growth state or with known levels of PKC activity, thus our data do not support a role for MT in HL‐60 differentiation or for PKC in MT expression.