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Rapid preparation of the nicotinic acetylcholine receptor for crystallization in detergent solution
Author(s) -
Hertling-Jaweed Sigrid,
Bandini Giampiero,
Müller-Fahrnow Anke,
Dommes Veronika,
Hucho Ferdinand
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81024-x
Subject(s) - torpedo , acetylcholine receptor , nicotinic agonist , alpha 4 beta 2 nicotinic receptor , crystallization , ganglion type nicotinic receptor , chemistry , yield (engineering) , receptor , acetylcholine , chromatography , nicotinic acetylcholine receptor , electric organ , biochemistry , biophysics , materials science , biology , endocrinology , organic chemistry , metallurgy
A novel rapid purification method for the nicotinic acetylcholine receptor from Torpedo electric tissue was developed. It allows preparation of 10 mg quantities of pure and stabile receptor protein within 2 days. This protein is used for crystallization attempts. Conditions are described which reproducibly yield crystals.