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The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line
Author(s) -
Doostdar H.,
Duthie S.J.,
Burke M.D.,
Melvin W.T.,
Grant M.H.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81021-4
Subject(s) - hep g2 , composition (language) , cell culture , enzyme , cytochrome , biochemistry , oxidase test , human liver , cytochrome p450 , biology , glutathione , chemistry , in vitro , genetics , linguistics , philosophy
When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10–20% of the cytochrome P‐450‐dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP‐glucuronyltransferase and glutathione‐S‐transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O ‐dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50‐, 30‐ and 10‐fold, respectively.