Premium
Isolation and characterisation of GLP‐1 7–36 amide from rat intestine Elevated levels in diabetic rats
Author(s) -
Kreymann B.,
Yiangou Y.,
Kanse S.,
Williams G.,
Ghatei M.A.,
Bloom S.R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)81008-1
Subject(s) - streptozocin , ileum , chemistry , medicine , gastrointestinal tract , glucagon like peptide 1 , arginine , high performance liquid chromatography , endocrinology , peptide , biochemistry , chromatography , diabetes mellitus , amino acid , type 2 diabetes , streptozotocin
Glucagon‐like peptide‐1 (GLP‐1) was purified to homogeneity by HPLC and anion‐exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP‐1 7–36 NH 2 , providing evidence that amidation occurs at an arginine residue during the post‐translational processing of GLP‐1. The distribution of GLP‐1 7–36 NH 2 ‐like immunoreactivity (GLP‐1 7–36 NH 2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin‐induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP‐1 7–36 NH 2 IR in the colon.