Premium
Demonstration of immunochemical identity between the synaptic vesicle proteins synaptin and synaptophysin/p38
Author(s) -
Gaardsvoll Henrik,
Obendorf Dieter,
Winkler Hans,
Bock Elisabeth
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80997-9
Subject(s) - synaptophysin , synaptic vesicle , synaptotagmin 1 , identity (music) , vesicle , chemistry , microbiology and biotechnology , biochemistry , biology , immunohistochemistry , physics , immunology , membrane , acoustics
The synaptic vesicle proteins synaptin and synaptophysin/p38 were shown to be immunochemically identical. Western immunoblot analysis of Triton X‐100 extracts from rat brain showed that polyclonal polyspecific anti‐synaptin antibodies and monoclonal antibody SY38 against synaptophysin both reacted with a band of 38 kDa. In two‐dimensional immunoblots of chromaffin granule membranes from bovine adrenal medulla anti‐synaptin and anti‐synaptophysin antibodies also recognized the same component. Finally, in a Western immunoblotting experiment SY38 reacted with an immunoisolated synaptin antigen.