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Effect of hydrocortisone on sialyltransferase activity in the rat small intestine during maturation Changes along the villus‐crypt axis and in fetal organ culture
Author(s) -
Kolínská Jiřina,
Ivanov Svetoslav,
Chelibonova-Lorer Heny
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80984-0
Subject(s) - crypt , sialyltransferase , organ culture , biology , endocrinology , hydrocortisone , medicine , small intestine , glucocorticoid , fetus , in vitro , biochemistry , pregnancy , sialic acid , genetics
Sialyltransferase activity was assayed in rat intestinal cells isolated as fractions reflecting the villus‐crypt axis of differentiation. In 13‐day‐old rats both endo‐ and exogenous sialyltransferase activity reached their maximum in undifferentiated crypt cells and their peaks overlapped. In contrast, sialyltransferase of the adult intestine was 4‐fold lower than that of sucklings in the crypts, with slight tendency to be transferred to the villus cells. Hydrocortisone applied to 10‐day‐old rats caused three days later a precocious drop of sialyltransferase activity in the crypt cells. Unlike in vivo, glucocorticoid responsiveness was accompanied by increased sialyltransferase activity in fetal small intestine cultivated for 17 days.