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Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO‐12049 in E. coli
Author(s) -
Aoyama Shigeyuki,
Yoshida Naoyuki,
Inouye Satoshi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80980-3
Subject(s) - lipase , pseudomonas , cloning (programming) , nucleic acid sequence , gene , microbiology and biotechnology , chemistry , molecular cloning , biochemistry , peptide sequence , escherichia coli , biology , enzyme , bacteria , genetics , programming language , computer science
The lipase gene from Pseudomonas fragi IFO‐12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of M r 29 966, which was close to the value of the lipase expressed in E. coli.