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Location of DNA‐binding segment of a positive regulator, OmpR, involved in activation of the ompF and ompC genes of Escherichia coli
Author(s) -
Tate Shin-ichi,
Kato Masashi,
Nishimura Yoshifumi,
Arata Yoji,
Mizuno Takeshi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80978-5
Subject(s) - escherichia coli , regulator , gene , dna , biology , genetics , escherichia coli proteins , microbiology and biotechnology
OmpR protein is a positive regulator involved in activation of the ompF and ompC genes which encode the major outer membrane proteins OmpF and OmpC, respectively. OmpR protein is considered to have a two‐domain structure. In the present study we isolated a C‐terminal fragment of the OmpR molecule, which bound to a specific promoter sequence of the ompF gene. We conclude that the C‐terminal portion of the OmpR protein contains a DNA‐binding site.