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Mitochondrial ribosomes of yeast: Isolation of individual proteins and N‐terminal sequencing
Author(s) -
Graack Hanns-Rüdiger,
Grohmann Lutz,
Choli Theodora
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80975-x
Subject(s) - ribosome , ribosomal protein , mitochondrial ribosome , yeast , biochemistry , ribosomal rna , eukaryotic large ribosomal subunit , protein subunit , saccharomyces cerevisiae , biology , eukaryotic ribosome , homology (biology) , gel electrophoresis , amino acid , chemistry , microbiology and biotechnology , rna , gene
Proteins of the small and large subunits of mitochondrial ribosomes from the yeast Saccharomyces cerevisiae were isolated and characterized by two‐dimensional gel electrophoresis. Ribosomal proteins of the large subunit were separated by reverse‐phase HPLC and up to 37 amino acid residues of the N‐terminal sequences of L3, L4, L9 and L31 were determined. No significant homology to ribosomal protein sequences so far determined from other organisms was found.