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Nerve growth factor increases the cyclic GMP level and activates the cyclic GMP phosphodiesterase in PC12 cells
Author(s) -
Laasberg Tiit,
Pihlak Arno,
Neuman Toomas,
Paves Heiti,
Saarma Mart
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80953-0
Subject(s) - cyclic gmp , nerve growth factor , phosphodiesterase , gtp' , second messenger system , ibmx , pde10a , endocrinology , medicine , chemistry , phosphodiesterase 3 , cgmp specific phosphodiesterase type 5 , biology , receptor , enzyme , biochemistry , nitric oxide , forskolin , erectile dysfunction
Nerve growth factor (NGF) rapidly increases the cyclic GMP (cGMP) level about 2–3‐fold and enhances the cGMP phosphodiesterase (PDE) activity about 2‐fold in rat pheochromocytoma PC12 cells. No changes in the level of cyclic AMP (cAMP) and in the activity of cAMP PDE were found. GTP and a nonhydrolysable analog of GTP, GMP‐PCP, at 100 μM, were able to mimic the effect of NGF on the cGMP PDE activity. These results suggest that the cGMP system may be one of the second messengers of NGF action in PC12 cells.