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Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase in human erythrocytes
Author(s) -
Kimura Hirokazu,
Nakano Minoru
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80949-9
Subject(s) - chemiluminescence , superoxide dismutase , hypoxanthine , chemistry , xanthine oxidase , superoxide , dismutase , biochemistry , microbiology and biotechnology , luminol , xanthine , enzyme , chromatography , biology
Superoxide dismutase in human erythrocytes was assayed by the inhibition of highly diluted erythrocyte lysates on 2‐methyl‐6‐( p ‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐ a ]pyrazin‐3‐one‐dependent luminescence induced by the hypoxanthine‐xanthine oxidase system. Our chemiluminescence procedure gave 95‐times higher sensitivity than the cytochrome c method. The concentration of superoxide dismutase in erythrocytes of Down's syndrome patients was approx. 1.8‐times higher than that of normal humans.