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Levels of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma reflect rates of bile acid synthesis in man
Author(s) -
Axelson M.,
Aly A.,
Sjövall J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80944-x
Subject(s) - cholestyramine , bile acid , medicine , chemistry , cholestasis , ursodeoxycholic acid , gastroenterology , cirrhosis , chromatography , endocrinology , cholesterol
A method for analysis of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma is described. Following solid‐phase extraction/purification the compound is determined by high‐performance liquid chromatography using a UV detector. The median concentration in healthy subjects was 12 ng/ml (range 3–40). The levels were lower in diseases associated with a low bile acid production: extrahepatic cholestasis, <1.5 ng/ml (range < 0.9–3); liver cirrhosis <1.5 ng/ml (range < 0.9–38), and higher in diseases associated with a high bile acid production: cholestyramine treatment, 188 ng/ml (range 54–477); ileal resection 397 ng/ml (range 128–750). The levels were essentially normal in patients with colon resection. The results are consistent with a strong positive correlation between the levels of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma and the rate of bile acid synthesis.