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A cytoplasmic‐membrane protein repressible by ammonium in Synechococcus R2: altered expression in nitrate‐assimilation mutants
Author(s) -
Madueño Francisco,
Vega-Palas Miguel A.,
Flores Enrique,
Herrero Antonia
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80936-0
Subject(s) - mutant , nitrate , ammonium , cytoplasm , biochemistry , nitrogen assimilation , biology , ammonium nitrate , assimilation (phonology) , protein expression , membrane , chemistry , gene , ecology , linguistics , philosophy , organic chemistry
A protein of ∼48 kDa was present in the cytoplasmic membrane of cells of Synechococcus R2 grown on nitrate or incubated in the absence of combined nitrogen, but not in ammonium‐grown cells. The level of this polypeptide was diminished in mutant strains that also exhibit reduced levels of other components of the nitrate‐reductive system. On the other hand, a mutant constitutive for nitrate uptake also synthesized constitutively the 48 kDa protein.