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Cleavage of specific sites of RNA by designed ribozymes
Author(s) -
Koizumi Makoto,
Iwai Shigenori,
Ohtsuka Eiko
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80935-9
Subject(s) - ribozyme , cleavage (geology) , ligase ribozyme , vs ribozyme , rna , mammalian cpeb3 ribozyme , chemistry , hairpin ribozyme , microbiology and biotechnology , computational biology , stereochemistry , biology , biochemistry , gene , paleontology , fracture (geology)
Two ribozymes were designed for site‐specific cleavage of RNA. A UA site in an undecaribonucleotide was cleaved by a ribozyme consisting of two partially paired oligoribonucleotides with chain lengths of 19 and 15. The other ribozyme, which consists of a 19‐mer and a 13‐mer, recognized a UC sequence at positions 42 and 43 of 5 S rRNA.