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Biosynthesis of psoralens Psoralen 5‐monooxygenase activity from elicitor‐treated Ammi majus cells
Author(s) -
Hamerski Daria,
Matern Ulrich
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80930-x
Subject(s) - bergapten , psoralen , elicitor , biosynthesis , microsome , monooxygenase , biochemistry , methoxsalen , chemistry , endoplasmic reticulum , biology , in vitro , enzyme , cytochrome p450 , dna , immunology , psoriasis
Microsomes prepared from cultured Ammi majus cells that had been challenged for 14 h with an elicitor derived from the cell walls of Phytophthora megasperma f.sp. glycinea (Pmg) converted psoralen to bergaptol (5‐hydroxypsoralen) in the presence of NADPH and oxygen. The enzymatic activity was characterized as an inducible cytochrome‐P‐450‐dependent monooxygenase associated with the endoplasmic reticulum. All of the steps involved in bergapten (5‐methoxypsoralen) biosynthesis in Ammi majus have now been demonstrated in vitro. The results suggest that bergaptol and not hydroxymarmesin in the precursor of bergapten.