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The binding of in vitro synthesized adenovirus DNA binding protein to single‐stranded DNA is stimulated by zinc ions
Author(s) -
Vos Hans L.,
van der Lee Frédérique M.,
Sussenbach John S.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80927-x
Subject(s) - dna , in vitro , zinc , chemistry , dna binding protein , single stranded binding protein , zinc finger , microbiology and biotechnology , biochemistry , biology , gene , transcription factor , organic chemistry
We have synthesized wild type DNA binding protein (DBP) of adenovirus type 5 (Ad5) and several truncated forms of this protein by a combination of in vitro transcription and translation. The proteins obtained were tested for binding to a single‐stranded DNA‐cellulose column. It could be shown that for binding of in vitro synthesized wild type DBP, it is necessary to add zinc ions to the in vitro translation system. Binding studies with the truncated proteins revealed that deletion of the carboxyl‐terminal 46 amino acids abolishes DNA binding.