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Isolation and characterization of a fraction from brain that inhibits 1,4‐[ 3 H]dihydropyridine binding and L‐type calcium channel current
Author(s) -
Janis R.A.,
Shrikhande A.V.,
Johnson D.E.,
McCarthy R.T.,
Howard A.D.,
Greguski R.,
Scriabine A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80923-2
Subject(s) - nitrendipine , dihydropyridine , chemistry , fraction (chemistry) , acetonitrile , chromatography , calcium channel , biophysics , calcium , biology , organic chemistry
Bovine brain was subjected to acid extraction and several purification steps. A fraction from brain that eluted from C18 reverse‐phase columns at 30–35% acetonitrile inhibited [ 3 H]nitrendipine binding to cardiac membranes. Further purification of this fraction on a sizing column in the presence of 40% acetonitrile yielded a low molecular mass fraction (< 1 kDa) that produced a time‐ and voltage‐dependent inhibition of L‐type (but not T‐type) Ca 2+ ‐channel current in GH 3 cells. The results suggest that this fraction contains an endogenous substance that binds directly to slowly‐inactivating Ca 2+ channels and thereby inhibits current flow.