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Estrogen‐controlled gene expression in tissue culture cells by zearalenone
Author(s) -
Mayr Ulrich E.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80921-9
Subject(s) - zearalenone , chloramphenicol acetyltransferase , estrogen , estrogen receptor , cell culture , transfection , mycotoxin , biology , gene expression , in vitro , chemistry , microbiology and biotechnology , gene , reporter gene , biochemistry , endocrinology , genetics , cancer , breast cancer
In two estrogen‐sensitive cell lines, Le42 and MCF‐7, the estrogenic potential of the nonsteroidal mycotoxin zearalenone has been investigated. The chloramphenicol acetyltransferase (CAT) gene expression in Le42 cells is induced by zearalenone after transfection with a CAT‐gene construct controlled by an estrogen responsive element [(1986) Cell 46, 1053–1061]. In MCF‐7 cells zearalenone induces at least 2 exoproteins (52 and 160 kDa) which are estrogen‐specific [(1980) Cell 20, 353–362). These data suggest that zearalenone acts by activating the estrogen receptor. Due to the high sensitivity of these cell lines for zearalenone both test systems are proposed as assays for a quantitative estimation of the biological (estrogenic) activity of this widespread mycotoxin.