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Expression of human cathepsin B protein in Escherichia coli
Author(s) -
Chan Marion Man-Ying,
Fong Dunne
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80920-7
Subject(s) - immunoscreening , cathepsin h , cathepsin o , cathepsin l1 , microbiology and biotechnology , cathepsin a , cathepsin b , escherichia coli , complementary dna , cathepsin l , biology , cathepsin e , cathepsin , cdna library , biochemistry , enzyme , gene
A cDNA fragment containing the coding sequence for the mature enzyme of human lysosomal proteinase cathepsin B was inserted in the pET plasmid expression vectors, so that it was placed under the control of transcription and translation signals from bacteriophage T 7 . Upon induction, cathepsin B antigen was detected by in situ immunoscreening of lysed E. coli and by Western blot analysis of bacterial lysates. To our knowledge this is the first report of abundant synthesis of cloned cathepsin B in any expression system. Subfragments of cathepsin B can also be generated by this technique and will be used to study cathepsin B structure and function.