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Calcium‐dependence of chromogranin A‐catecholamine interaction
Author(s) -
Westermann R.,
Stögbauer F.,
Unsicker K.,
Lietzke R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80917-7
Subject(s) - chromogranin a , calcium , chromaffin cell , chemistry , endocrinology , catecholamine , medicine , granule (geology) , organelle , vesicle , adrenal medulla , biochemistry , biology , immunohistochemistry , membrane , paleontology , organic chemistry
Major components of the secretory organelle of bovine adrenal medullary cells, the chromaffin vesicles, are the acidic protein chromogranin A, catecholamines and Ca 2+ . The binding of Ca 2+ to chromogranin A has been established. To study the interaction between chromogranin A and catecholamines and its dependence on Ca 2+ we immobilized chromogranin A to a newly raised monoclonal antibody. It is shown that chromogranin A can bind (i) about 0.5 mol catecholamines per mol in a non‐calcium‐dependent manner and (ii) about 5 mol per mol in the presence of calcium. These results further support the notion that chromogranin A may act as a secretory granule‐condensing protein.