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Occurrence of glucose polymer in undifferentiated PC12 cells
Author(s) -
Rasilo Maija-Liisa,
Yamagata Tatsuya
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80896-2
Subject(s) - concanavalin a , pronase , chemistry , monosaccharide , polymer , galactose , biochemistry , sepharose , glycogen , chromatography , agarose , enzyme , trypsin , organic chemistry , in vitro
A large glucose polymer was found, following pronase digestion, in PC12 pheochromocytoma cells metabolically labeled with [1‐ 3 H]galactose. The polymer was excluded from a Bio‐Gel A‐0.5 m column and adsorbed by immobilized concanavalin A‐Sepharose from which it was eluted with 10 mM α‐methylmannoside. Glucose was found to be the sole component monosaccharide. Except for those capable of degrading glycogen, no exo‐ or endo‐glycosidases cleaved the polymer. This is the first report on the occurrence of a glucose polymer in undifferentiated PC12 cells.