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A methyltransferase for synthesis of the phytoalexin 6‐methoxymellein in carrot cells
Author(s) -
Kurosaki Fumiya,
Nishi Arasuke
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80894-9
Subject(s) - daucus carota , phytoalexin , elicitor , chemistry , coumarin , methylation , methyltransferase , moiety , biochemistry , methionine , enzyme , stereochemistry , botany , biology , organic chemistry , amino acid , gene , resveratrol
Carrot ( Daucus carota L.) roots treated with 2‐chloroethylphosphonic acid or uronide elicitor accumulated the phytoalexin 6‐methoxymellein. The extracts of these cells catalyzed methylation of 6‐hydroxymellein to 6‐methoxymellein with S ‐adenosyl‐L‐methionine as a methyl donor. Activity of the O ‐methyltransferase was not found in fresh carrot roots but increased when they were treated with either type of elicitor in parallel with the increase in 6‐methoxymellein content. The enzyme did not methylate 6‐methoxymellein, 3,4‐dehydro‐6‐methoxymellein, and some coumarin derivatives, though 3,4‐dehydro‐6‐hydroxymellein accepted methyl moiety at the same rate as in 6‐hydroxymellein.