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Effect of the bile acid taurolithocholate on cell calcium in saponin‐treated rat hepatocytes
Author(s) -
Combettes Laurent,
Dumont Micheline,
Berthon Brigitte,
Erlinger Serge,
Claret Michel
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80889-5
Subject(s) - neomycin , endoplasmic reticulum , chemistry , saponin , vasopressin , calcium , hepatocyte , endocrinology , microbiology and biotechnology , medicine , biology , biochemistry , in vitro , pathology , alternative medicine , organic chemistry , antibiotics
Neomycin was used to assess the involvement of Ins (1,4,5)P 3 in the Ca 2+ release from the endoplasmic reticulum induced by the bile acid taurolithocholate. In saponin‐permeabilized rat hepatocytes, neomycin via its ability to bind Ins (1,4,5)P 3 abolished the release of Ca 2+ induced by added Ins (1,4,5)P 3 . In contrast, it did not alter the Ca 2+ release initiated by the bile acid. In intact cells, neomycin had no effect on the [Ca 2+ ] i rises promoted by taurolithocholate and vasopressin. It is suggested that the effect of taurolithocholate in liver is not mediated by Ins (1,4,5)P 3 but results from a primary action on endoplasmic reticulum.