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Dissociation between intracellular calcium mobilization and insulin secretion in isolated rat islets of Langerhans
Author(s) -
Morgan Noel G.,
Hurst Roger D.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80887-1
Subject(s) - carbachol , endocrinology , medicine , bombesin , islet , extracellular , intracellular , calcium , calcium in biology , insulin , insulin oscillation , secretion , chemistry , stimulation , cholinergic , secretagogue , biology , neuropeptide , receptor , biochemistry
The neuropeptide bombesin provoked a dose‐dependent stimulation of 45 Ca 2+ efflux from pre‐loaded islets of Langerhans. This response occurred rapidly, was not sustained and did not depend on the presence of extracellular calcium, suggesting that it resulted from the mobilization of intracellular calcium stores. Under conditions when large increases in 45 Ca 2+ efflux were observed, bombesin completely failed to stimulate the rate of insulin secretion. Similar results were also obtained with the muscarinic cholinergic agonist, carbachol. The data suggest that the release of calcium from intracellular pools is not sufficient to induce an increase in insulin secretion in normal islet cells.