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Synthesis of a photoaffinity‐spin‐labeled derivative of ATP and its first application to F 1 ‐ATPase
Author(s) -
Vogel-Claude Pia,
Schäfer Günter,
Trommer Wolfgang E.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80878-0
Subject(s) - covalent bond , nitrene , chemistry , moiety , derivative (finance) , atpase , stereochemistry , photoaffinity labeling , ribose , enzyme , biochemistry , binding site , organic chemistry , financial economics , economics , catalysis
The synthesis of an ATP derivative is described, in which a spin‐label is attached to the 3′‐position of the ribose moiety and an azido group to C8 of the adenine ring (SL‐N 3 ‐ATP). Irradiation of this compound at 350 nm generates a nitrene, which will react with any functional group in its vicinity. SL‐N 3 ‐ATP exhibits a strongly immobilized ESR spectrum in a complex with F 1 ‐ATPase from beef heart mitochondria. It was covalently incorporated into this enzyme. SL‐N 3 ‐ATP may be employed in ESR investigations under conditions in which non‐covalent interactions are too weak for motionally restricted species to be easily observed.