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The 5′‐CA DNA‐sequence preference of daunomycin
Author(s) -
Skorobogaty Andrew,
White Robin J.,
Phillips Don R.,
Reiss James A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80877-9
Subject(s) - dna , footprinting , sequence (biology) , nucleotide , consensus sequence , microbiology and biotechnology , nucleic acid sequence , transcription (linguistics) , dna sequencing , rna polymerase , binding site , biology , deoxyribonuclease i , dna footprinting , biochemistry , chemistry , rna , transcription factor , gene , dna binding protein , base sequence , linguistics , philosophy
The DNA‐sequence specificity of daunomycin was investigated by DNase I footprinting and an E. coli RNA polymerase transcription‐inhibition assay. The 5′‐CA sequence was identified as being the highest affinity binding site, although other modest affinity (5′‐GC, CG, CT, TC, AC) and poor affinity sites (5′‐AA, AT, TA) were also observed. The preference of daunomycin for 5′‐CA nucleotide sequence suggests that its biological activity may arise from association with the 5′‐CA‐containing sequences thought to be associated with genetic regulatory elements in eukaryotes.