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A mutant LexA repressor harboring a cleavage motif cysteine‐glycine remains inducible
Author(s) -
Granger-Schnarr Michèle,
Oertel Pascale,
Schnarr Manfred
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80866-4
Subject(s) - repressor lexa , repressor , mutant , cysteine , psychological repression , chemistry , amino acid , microbiology and biotechnology , biology , biochemistry , gene , gene expression , enzyme
Using site‐directed mutagenesis of the lex A gene we have changed the amino acid Ala‐84 of the LexA repressor for a cysteine. The reason for this change was the striking homology between LexA and UmuD and the comparable size of the two amino acid side chains. Using an in vivo repression/induction assay it is shown that the LexA‐Cys‐84 mutant remains inducible by mitomycin C and UV irradiation essentially in the same way as the wild‐type repressor.